An F41-K88-related genetic determinant of bovine septicemic Escherichia coli mediates expression of CS31A fimbriae and adherence to epithelial cells

Purification and characterization of P fimbriae from an Escherichia coli strain isolated from a septicemic turkey.

A pap+ Escherichia coli isolate from a turkey with colisepticemia expressed P fimbriae with a major subunit of an apparent molecular mass of 18 kDa which reacted with anti-F11 serum. This fimbriae was purified and polyclonal antiserum was produced in rabbits. The N-terminal amino acid sequence of the major fimbrial subunit of the avian P fimbriae was identical to that of F11. On immunoblotting,...

Adherence of diarrheagenic Escherichia coli strains to epithelial cells.

Nucleotide sequence of the F41 fimbriae subunit gene in Escherichia coli B41.

A clone encoding the F41 f imbnal sub-unit gene from E coli B41, was isolated from an B1BL3 library using an oligonucleotide based on the published N-termlnal amino acid sequence (1). The DNA sequence of an t I66bp fragment revealed an open reading frame encoding a protein of 277 amino acids including a 22 amino acid leader sequence (dash underlined). This corresponds to a mature protein of 290...

Structural studies on the Escherichia coli O101 lipopolysaccharide found in association with F41 and K99 fimbriae.

In this study it was shown that the O101 lipopolysaccharide isolated from Escherichia coli B41 did not contain an O-specific polysaccharide and that its sugar moiety is probably restricted to the core oligosaccharide. It is characterized by the presence of galactose, glucose, N-acetylglucosamine, heptose and 3-deoxy-D-manno-2-octulosonic acid and the fatty acid composition is typical of an Ente...

Aggregative adherence fimbriae I of enteroaggregative Escherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes.

Strains of enteroaggregative Escherichia coli (EAggEC) have been implicated in several studies as important agents of persistent diarrhea among infants in the developing world. We have previously shown that the aggregative adherence (AA) property of EAggEC is associated with the presence of a 60-MDa plasmid which confers AA when introduced into E. coli HB101. Here, we report the cloning of the ...